Can I use a pan phosphatase inhibitor directly into a cell culture to treat cells before treating cells with a DNA damaging agent like UV?

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How to Genotyping of point mutation mouse?

Hi guys, We introduce some mice with two adjacent point mutation of specific molecules, however, it is difficult to carry out genotyping of this mice. DNA base pair and protein size is similar...

24 January 2024 9,958 1 View

When doing the ADP-Glo kinase assay, will the incubation time affect the experimental results?

Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...

27 December 2023 8,915 2 View

Peek fitting to sus ?

Hi, For some technical question. I have old HPLC machine, For HPLC, detector was died, so I'm going to use it for dual head general pump not for analytics. This machine pump's out line...

12 September 2023 4,627 0 View

Water uptake isotherm P/Po = RH ?

Hi, I have a question about isotherm. I'm recently researching MOF especially water sorption. I use ASAP 3 Flex, and the result is P/Po as x-axis. Is it same meaning of RH% ? (relative...

07 June 2023 3,007 0 View

Can you teach me about polycistronic vector cloning and overlap PCP using 2A sequence?

Hello, I'd like to ask for your help because polycystronic vector cloning is not working well using 2A sequence. The final product we want to make is as follows restriction enzyme1 - Target1 -...

21 May 2023 6,328 2 View

Why does my FRF oscillate?

Hello. I have question about the FRF result. I performed the vibration test and got some FRF data. As can be seen the following figure, the FRF result is oscillated from 70Hz to 75Hz. This...

20 May 2023 2,243 3 View

How to do DIp Coating for MOF ?

Hello, I would like to do dip coating for MOF material on fiber, cellulose and so on. MOF is kind of very fine power, and It was very difficult to do coating. Could you advise ? and Any coating...

26 April 2023 9,209 1 View

What is the formula for the change in viscosity with concentration?

Given the viscosity of a solution of a known concentration, can the change in viscosity when the concentration changes be calculated by a formula?

02 April 2023 6,816 5 View

What are some commonly used staining reagents for staining the cell membrane of endothelial cells?

Dear community, Recently, I'm doing an experiment to measure the permeability of endothelial cells(HUVEC, HPAEC). I understand that it is important to make sure that endothelial cells form...

14 March 2023 7,689 2 View

Why is it good to have a uniform shape of beads in a fixed bed column?

Beads have been widely used in columns from the past. Many studies have been conducted to make the beads into a uniform shape. However, it is difficult to find relevant literature. 1. Why should...

22 October 2022 8,034 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View