I gave 3 chloroform extraction steps still it is not leaving from Rna and after Dnase treatment also Dna is not degrading please let me what would be the problem.
we use Qiagen RNeasy kit for purification of TB RNA. It has gDNA eliminator column. after purification we give DNase treatment using kit ambion DNase kit. RNA comes out very pure. Good luck.
If your RNA sample has either organic or protein contamination DNase might not work. Also, during collection of aqueous phase don't be greedy to collect all the supernatant. Don't overload TRIzol with cells.