Ravi Prasad

4 Questions 7 Answers 0 Followers

Questions related from Ravi Prasad

Why monocytes adhere while differentiating them as Dendritic cells?

I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?

10 November 2023 8,319 2 View

How to extract membrane proteins from human macrophages?

later in down stream i have to use these proteins for pulldown. so kindly address me how to extract them in native form ? thank you regards ravi

05 April 2022 3,074 2 View

How to block Toll like receptors with antibodies in thp1 macrophages?

I want to block TLR1 and look for downstream molecules, so kindly let me know how to do this assay with what concentration of antibody it would be the right. thank you.

11 April 2021 820 4 View

How to get ride of DNA contamination in RNA preparation from M.smegmatis?

I gave 3 chloroform extraction steps still it is not leaving from Rna and after Dnase treatment also Dna is not degrading please let me what would be the problem.

20 February 2017 7,487 5 View