I have been making a method to determine chlorhexidine using an LC-MSMS (ESI+ modus).

My solutions are made in acetonitril/water (65/35) and 0,2% formic acid is added to ionize the compound. My eluens consists of the same chemicals as the solution for my analyte.

Now I got a problem of tailing peaks. I am using a Acquity UPLC BEH Columns.

The cause for the tailing is most likely the low pH that is used (pH +/- 3). however I cant work at a higher pH since I need the added acid for good detection using MS detector.

Changing the column is not a possibility either (budget reasons).

Any Suggestions?

Kind Regards

Bertijn

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