Chemical fixation vs heat killing of bacteria: which method is better to preserve MAMPs?

Hello Scientific World, I am working on host-microbe interaction specifically bacterial stimulation of macrophages. In my in vitro experiments, I would like to use dead but intact bacteria to...

05 June 2018 7,558 2 View

What is an accepted equivalent of non-parametric version two-way ANOVA in R package?

I use R for my statistical analysis. I want to analyse a kinetics experiment that involves measurement over 3 time points with control, genetic background 1, 2 and 3. Can anyone suggest a test and...

09 October 2016 8,503 4 View

How to differentiate HL-60 to Neutrophils in suspension and which markers to check ?

I have started working on HL-60 cell lines. My aim is to look for Calcium flux upon activation, chemotaxis and also infection with bacteria. I followed Dr. Steven Ackerman's protocol for thawing...

04 May 2016 9,921 11 View

Can anyone suggest cell lines to study cytokines production upon infection in vitro ?

08 September 2015 10,013 5 View

What's the best way to detach primary macrophages from surface of tissue culture plates/dishes ?

I have started working with primary human and murine macrophages and have successfully differentiated and used for infection experiments. I perform mostly CFU assays with bacteria. Although, I...

03 April 2015 3,675 16 View

What are major inflammatory cytokines that can be quantified upon lung infection ?

I am testing a bacterial mutant in pneumonia model and also wanted to check for inflammation. Apart from microscopic examination using HE staining, I wanted to quantify inflammatory cytokines in...

02 March 2014 5,520 6 View

Which is the best method for elution of DNA (biotin tagged) from Streptavidin Magnetic Beads?

I have primers with Biotin tag. I use them in PCR for enrichment of particular amplicons. I have a feeling that the 6M Guanidine HCL recommended is not doing the best job. I also have to use the...

04 May 2013 5,702 7 View

DNA eluted with 6M Guanidine hydrochloride from Streptavidin Magnetic beads, does it need further purification for downstream applications?

1. Will be compatible with Agarose TAE gels, when loaded directly? 2. Will it be compatible in Phusion PCR reactions, further as template? Note: The manufacturer recommends 6m GuHCL for elution...

04 May 2013 8,204 7 View

How to check differentiation efficiency of Human monocyte/macrophage from HL-60 cells?

Hello fellow researchers, Quite recently, we differentiated HL-60 cells into PMNs using DMSO and had a fair amount of success. I usually check for the increase in surface CD11b and also control...

01 January 1970 2,453 5 View

Why does THP-1 cells shown different populations in flow cytometry scatter plot?

Hello Scientific World, I would highly appreciate some help here. I am using THP-1 monocytes as my pet cell line to generate human macrophages. Before every differentiation process, I test my...

01 January 1970 1,379 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View