Hello Scientific World,
I am working on host-microbe interaction specifically bacterial stimulation of macrophages. In my in vitro experiments, I would like to use dead but intact bacteria to trigger macros i.e. exploiting only Microbe-associated molecular patterns (MAMPs) on surface.
My 96-well plate assays demand a method of inactivating bacteria that is time-efficient and highly parallel.
Which method works better in such a case ?
I would be really grateful for any incoming suggestions,
Thanks
I worked with Brucella abortus for my Ph.D. and we had a hard time getting the bacteria to stick to microscope slides even with heat fixation. What really worked best was to smear the bacteria on a slide and let them air-dry in a 37 C incubator before staining.
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