4 Questions 38 Answers 0 Followers
Questions related from Guanfan Mao
Sometimes two genes contain high Homology[95~98%],it is difficult for Real Time PCR (SYBR Green method)to design primers,and it seems not possibile to distinguish the Major gene .Can somebody...
12 December 2012 9,550 4 View
Sometimes the whole gene region contains high GC content[~80%],it is difficult for Real Time PCR to analyze the simples;I have tried to design several primers to optimizate the results,but they...
12 December 2012 8,106 6 View
I want to know whether by the way of Real Time PCR can distinguish the target gene's expression level Between the RNAi Transgenic lines and WT, if can do, how to do?
12 December 2012 5,314 2 View
Sometimes two plant genes (A and B) contain high Homology[95~98%],whether can use RNAi to Downregulate A gene at the same time but not affect on B.How to select the RNAi region?
12 December 2012 6,531 0 View
