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Questions related from Sudip Das
Hello Scientific World, I am working on host-microbe interaction specifically bacterial stimulation of macrophages. In my in vitro experiments, I would like to use dead but intact bacteria to...
06 June 2018 7,661 2 View
I use R for my statistical analysis. I want to analyse a kinetics experiment that involves measurement over 3 time points with control, genetic background 1, 2 and 3. Can anyone suggest a test and...
10 October 2016 8,602 4 View
I have started working on HL-60 cell lines. My aim is to look for Calcium flux upon activation, chemotaxis and also infection with bacteria. I followed Dr. Steven Ackerman's protocol for thawing...
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I want to study cytokine production upon S. aureus infection in vitro. I have the following concerns: 1. Which human/mouse cells are best suited ? 2. Which assay is best suited in terms of both...
09 September 2015 10,116 5 View
I have started working with primary human and murine macrophages and have successfully differentiated and used for infection experiments. I perform mostly CFU assays with bacteria. Although, I...
04 April 2015 3,935 16 View
I am testing a bacterial mutant in pneumonia model and also wanted to check for inflammation. Apart from microscopic examination using HE staining, I wanted to quantify inflammatory cytokines in...
03 March 2014 5,607 6 View
I want to use mouse tissue homogenates containing bacteria and plate it on agar plates. For my experimental purpose I need to pool the bacteria from the plates. If I scrape off the bacteria, will...
06 June 2013 2,626 2 View
1. Will be compatible with Agarose TAE gels, when loaded directly? 2. Will it be compatible in Phusion PCR reactions, further as template? Note: The manufacturer recommends 6m GuHCL for elution...
05 May 2013 8,324 7 View
I have primers with Biotin tag. I use them in PCR for enrichment of particular amplicons. I have a feeling that the 6M Guanidine HCL recommended is not doing the best job. I also have to use the...
05 May 2013 5,826 7 View
Hello fellow researchers, Quite recently, we differentiated HL-60 cells into PMNs using DMSO and had a fair amount of success. I usually check for the increase in surface CD11b and also control...
01 January 1970 2,561 5 View
Hello Scientific World, I would highly appreciate some help here. I am using THP-1 monocytes as my pet cell line to generate human macrophages. Before every differentiation process, I test my...
01 January 1970 1,478 6 View
Hello, I am currently trying to standardise qPCR primers for IL-10 expression in human macrophages i.e. checking amplification, primer efficiencies. Before the actual stimulation by bacteria, I...
01 January 1970 5,303 4 View