Hi,
I'm facing 2 issues with our LLC and B16/F10 melanoma model in order to count lung metastasis.
In order to get significant metastasis, we need to incubate LLC and B16/F10 in mice for about a month. (LLC administration iv (tail vein): 5x10^5 cells/mouse / B16-F10: 1x10^6 cells/mouse IV).
At that endpoint, we obtain huge variation from mouse to mouse within the same experimental group.
How do you reduce this variation inside a same experimental group?
Is there another reliable cancer model to adresse metastasis in the lungs aside LLC/B16-F10 or urethane-induced?
thanks in advance
Hi Alexis,
We usually perform an orthotopic cell engraftment, directly to the lungs. This leads to low variability from an animal to another regarding the lung tumour.
Here is a paper in which we described the procedure for the M109 lung carcinoma model. DOI 10.1016/j.ejpb.2021.04.018
Best
Rémi
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