How to get involved in research opportunity?

More Alessandra O Donaldson's questions See All

Could someone please provide me a PROTEIN extraction protocol from strawberry FRUIT?

Hi everyone, this topic is completely new to me, so i need some help to proceed with the easiest (if possible) method to achieve proteins extraction from strawberry fruits! Thanks in...

22 April 2024 2,609 0 View

How does a daughter obtain leadership in a family firm?

As consultant and a researcher I am very eager to study family firms so to help them succeed, as I believe they are a very important element of the economic and social (family, community)...

15 April 2024 7,422 0 View

What is the minimum amount of Polystyrene I can use to dissolve/dilute it in Ethyl Acetate or Acetone?

I am working on recycled cups made by Polystyrene and I am trying to dissolve them completely in Acetone and/or Ethyl Acetate solvent. However, it is quite hard to get it and I am not obtaining a...

25 March 2024 794 3 View

Whole spheroids immunofluorescence staining protocol?

Hi everyone! I want to perform an immunofluorescent staining of spheroids from human glioblastoma cells fixed in 4% PFA. I found many protocols and papers suggesting different solutions and times...

02 March 2024 4,617 2 View

How can I improve viability when thawing PBMCs with 20% DMSO? Is there a protocol that can separate the dead cells from the viable ones?

07 February 2024 8,893 4 View

How to analyze mitochondria morphology (shape), and measure mitochondria length and number in MitoTracker-stained cells?

I would like to analyze mitochondria morphology (shape), and measure mitochondria length and number in Mitotracker-stained HDFs cells (40X and 60X images). Is there an easy-to-use tool or software...

18 March 2022 3,681 4 View

What score range of the Geriatric Depression Scale determines a clinically meaningful change?

I would like to use the Geriatric Depression Scale to investigate the efficacy of a therapy treatment on depressive symptoms in people with dementia. I am aware of the cut-off and score range of...

28 February 2022 196 6 View

Why some antimicrobial assays keeps the drug in contact with the culture for less than 24 hours?

I know that 24, 48 and 72 are common but less than 24 hours is "allowed"? If my drug has some activity (low IC50) against some bacteria's strains in only 4 or 6 hours, is it enough to prove its...

26 January 2022 5,391 1 View

How can I get the accuracy of a microbiological assay, comparing a serial dilution of standard ATP and ATP released from E. coli?

I have been using a t test to do it, considering homoscedastic and two-tailed t test (acceptable level of more than 70%), but I don't know if it is appropriate... Also, I don't know how much...

05 December 2021 8,630 2 View

Does the GFP fluorescence disappear in fixed transfected cells?

I transfected my cells (primary human dermal fibroblasts) with a plasmid containing the gene of interest and a C-terminal turboGFP tag. I could see the turboGFP fluorescence using a fluorescence...

11 November 2021 7,272 3 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Why do exism movements become permanent dictatorship threats within liberal democracy thinking under majority rule-independent rule of law system?

Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...

04 August 2024 8,125 3 View

Adriani - Suwito

Greetings, I am happy to receive information about the joint research plan, but my expertise is in infectious and non-infectious epidemiology and I focus more on HIV/AIDS cases, which will be a bit difficult for me later, so I would like to thank you for the offer.

my regard

Adriani

Alessandra O Donaldson

Thank you for answering.