Hai, I am trying to isolate DNA from frozen and fresh fish samples for PCR.

For fresh samples i am getting a clear DNA band in the agarose gel, but in the case of frozen fish i am getting smear. The same problem occur in the PCR product also. How to get the good quality DNA from Frozen fish.

Here i attach those DNA and PCR product. freash samples give clear bands and frozen fish give smear.

Please give the suggestion to get rid of this problem.

Thank you

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