BT549- Tail vein injection?

Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...

14 October 2020 215 2 View

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods?

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods? if possible such as 1. Calorimetric or Spectroscopic methods and much more

26 August 2020 349 10 View

Suggest some journals for research proposal?

I have selected the topic Drought assessment using remote sensing and GIS. Please kindly recommend some journals for better understanding the topic.

15 June 2020 3,104 3 View

Recent topics in GIS and Remote sensing ?

I need a topic for my Major dissertation work in the field of GIS and Remote sensing . i want to show my programming language skills also. i need current innovative topic .Can anyone suggest me...

30 March 2020 2,761 26 View

Please point me to sites where I can find health related research papers ?

The challenge is to create a model that uses data from the first 24 hours of intensive care to predict patient survival . My dataset has more than 130,000 hospital Intensive Care Unit (ICU) visits...

23 February 2020 3,583 2 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

Conversion of ppm gor NH3 gas to micromole/g ?

I use ammonia of 0.3g in a TPD experiment. I get 21.02 ppm of NH3 which I intend to convert it to micromole/g of NH3. I have got some feedback from professors but it seems to be a little...

02 July 2019 4,828 5 View

What are the changes in the plasma during co-sputtering?

I am currently working on optimization of TiNi thin film deposition using magnetron sputtering. I was able to obtain a free standing film using a single target with the following parameters. Ar...

23 October 2018 782 7 View

Inhibitors of EGFR internalization?

In our lab, to block internalization and endocytosis of EGFR, an inhibitor solution of 2 mM of sodium fluoride (NaF), 10 mM of sodium azide (NaN3) and 5 mM of 2-deoxy-D-glucose is used. Someone...

08 October 2018 4,646 3 View

How to get free standing NiTi Thin films?

Hi all, I am trying to get a free standing NiTi by depositing a layer of copper between a Si substrate and the film but when I treat the substrate with HNo3 the film just breaks, I think the film...

20 August 2018 7,363 7 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View