Hello,
I am using BNL 1ME and have tried both subcu, orthotopic and portal vein injection in balb/c mouse. I dont see any tumors even 4 weeks after injection. I am seeing mixed publications online, some get great results and others need to do in vivo passages to develop highly metastatic cell lines. Does anyone have experience with that cell line in vivo or any ways to make it more invasive through doing transwell selection?
Thanks!