I am using LightCycler® 96 for absolute quantification, and want to generate a standard curve, as I am using one "Reference gene" with the 10 different samples with different cDNA dilutions such as 5x, 10x, 25 x,
But during the sample editor, there is an option "Concentration"
so which concentration will I have to write?
Keeping in mind the different dilution series ( 5x, 10x, 25 x, ) what will be the concentration for these dilutions?
Your dilution factors are : 5x, 10x and 25x
your concentration is a quantification of your cDNA from each dilution using a Nanondrop. You will get your concentration of cDNA sample ng/ul
hope it helps :)
Either choose for 2x or 10x dilution series of the template (known plasmid copies) covering a minimum of 5 points. This would be used of the generation of standard plot used in abs quantification.
Vipul Batra Thanks for the suggestion, you mean dilution series as 5x, 10x, 15x?
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