I have calculated ddCt for my qPCR where I have treated mice and checked for change in gene expression. Since, the treated and untreated were different I calculated dCt for each of them with respect to control gene and took and average of them and then, compared the treated to untreated. This gives me a single value for 5 different genes during treatment. I calculated the error propagation by taking the sqrt( sum of squares of errors). What is the best way to plot this? How to determine the statistical significance?
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