In ansys, how to find the deflection of beam?

07 November 2016 8,915 7 View

Controls in western blotting?

10 April 2016 4,678 3 View

Are there any reasons why HaCaT show the presence of Cx43 in the cytoplasm?

Cx43 is usually present on the plasma membrane of HaCaT cells. Immunofluorescence staining for Cx43 showed the staining in cytoplasm, which is wierd. I tried running western blots with total...

03 April 2016 6,164 1 View

How can I get the crystallographic card of Cloisite 93 A ( a modified Montmorillonite nanoclay) & epoxy/cloisite nanocomposites?

Crystallographic card (PDF) JCPDS indexes

14 September 2014 2,036 5 View

How to use azocoll as a substrate for determining recombinant serine protease?

I am working on recombinant subtilisin-like serine protease. As I have to analyze whether my recombinant protein of interest is active or not, I am using azocoll as substrate. I have used...

04 April 2014 5,394 2 View

Are there any alternative methods for in vitro release or how to find a particular wavelength for an oil in a capsule?

My encapsulated compound is essential oil. When I run it in UV I get lots of peaks which look like noise. They look the same even when diluted. In similar papers they report a particular...

18 March 2014 9,852 1 View

Any suggestions regarding multiple regression in SPSS?

My query is: 1. When we run a multiple regression it gives us a overall significance value for the model done taking into account all the independent variables (ANOVA table) 2. It then gives...

29 January 2014 894 10 View

Cell lysis buffer composition for his tagged protein purification in soluble fraction?

The protein I am working on is quite large (Approx 80kDa). I used the cell lysis buffer (PBS, PMSF and Triton X- 100) and finally I found my protein is present in the pellet. I am confused about...

04 June 2013 1,539 7 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View