Does oil interfere Total Dissolved Solid determination in water?

Dear friends, this is regarding Total Dissolved Solids test. when we have a oil mixed sample how do we analyze TDS? when sample is filtered through a glass micro fiber filter paper, oil in the...

04 November 2020 5,065 3 View

Conductivity value not matching with other parameters?

10 March 2019 2,401 3 View

In ansys, how to find the deflection of beam?

07 November 2016 8,915 7 View

Controls in western blotting?

10 April 2016 4,678 3 View

Are there any reasons why HaCaT show the presence of Cx43 in the cytoplasm?

Cx43 is usually present on the plasma membrane of HaCaT cells. Immunofluorescence staining for Cx43 showed the staining in cytoplasm, which is wierd. I tried running western blots with total...

03 April 2016 6,164 1 View

How can I get the crystallographic card of Cloisite 93 A ( a modified Montmorillonite nanoclay) & epoxy/cloisite nanocomposites?

Crystallographic card (PDF) JCPDS indexes

14 September 2014 2,036 5 View

How to use azocoll as a substrate for determining recombinant serine protease?

I am working on recombinant subtilisin-like serine protease. As I have to analyze whether my recombinant protein of interest is active or not, I am using azocoll as substrate. I have used...

04 April 2014 5,394 2 View

Are there any alternative methods for in vitro release or how to find a particular wavelength for an oil in a capsule?

My encapsulated compound is essential oil. When I run it in UV I get lots of peaks which look like noise. They look the same even when diluted. In similar papers they report a particular...

18 March 2014 9,852 1 View

Any suggestions regarding multiple regression in SPSS?

My query is: 1. When we run a multiple regression it gives us a overall significance value for the model done taking into account all the independent variables (ANOVA table) 2. It then gives...

29 January 2014 894 10 View

Cell lysis buffer composition for his tagged protein purification in soluble fraction?

The protein I am working on is quite large (Approx 80kDa). I used the cell lysis buffer (PBS, PMSF and Triton X- 100) and finally I found my protein is present in the pellet. I am confused about...

04 June 2013 1,539 7 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View