Is there any selective media to grow filamentous fungus only (not with yeast)?

22 November 2020 5,370 7 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

What is the pebbling number of a cycle graph with 4 vertices?

i need the number of pebbles for a cycle graph with 4 vertices

24 September 2019 4,188 1 View

Anyone know where the micro tensile testing machine available?

In Chennai where micro tensile testing machine available

19 August 2019 3,926 2 View

How do I etch nichrome thin film on polyimide(kapton) substrate? Can someone explain the procedure?

I'm currently working on fabricating etched heating element for a project work.

08 August 2018 1,746 3 View

Is there an association between non-vegetarianism and violence?

Is there any scientific study of this possible association? There have been informal discussions and surmises, but a serious study with appropriate controls to cancel out extraneous factors is...

29 May 2018 903 3 View

What could be the possible reasons for getting 20 unit less value of mass of pure compound which is well charecterised by NMR?

My compound is well charecterised by NMR but in case of ESI-mass spectrum it is coming 20 unit less then the expected mass. Molecule is not having any associatio with water. I could not find any...

12 September 2017 6,260 2 View

Different significance values on SPSS and PLS SEM. Please help.

On SPSS I am getting a result that shows no significant impact of the independent variable on the dependent. But when I am running the data on SmartPLS I am getting a significant impact. Please...

24 July 2017 5,505 3 View

Is gender a dichotomous variable? If yes, Can t-test be run on single large sample dichotomous variable (demographic)?

My colleague's thesis consists of a situation where she has performed a t-test to analyse the differences between male and female respondents' responses with respect to all the independent and...

19 July 2017 2,255 6 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View