I am trying to purchase a transilluminator for visualizing agaraose gels. filter sizes of instruments vary from 210 x 260 mm or 200 x 80 mm etc. Is this an indication of the size of gel that can be visualized? thanks.
Sharmila Jayasena
The UV transilluminator functions by emitting high quantities of UV light through the viewing surface. A UV transilluminator is most commonly used to see DNA and protein agarose and polyacrylamide gels following electrophoresis.
The transilluminator with a 312nm wavelength is appropriate for normal gel documentation needs. Transilluminators with a wavelength of 254nm induce greater DNA damage and are better suited for other purposes such as crosslinking. Because 254nm bulbs may be seen through the filter, they are inappropriate for gel documentation.
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