How to fragment DNA.

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Which software do you use to analyze loss-of-heterozygosity?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

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If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

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Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

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Full Enquiry On Biomass Energy In engineering Technology?

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Why does it show two bands when I add single hairpin structures to a gel?

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Muzaffer Arıkan

You can use Covaris, Caliper or Sage for fragmentation and size selection steps of Illumina sequencing. The detailed information about these instruments is given in the Illumina website below:

http://www.illumina.com/applications/sequencing/automation.ilmn

Domenico Vladimiro Libri

Ty! I have just found a Covaris to use.