Although the EC50 and IC50 values can be found by plotting concentration against response in excel but how to find the fiducial limit at 95% CI?
Use should use Probit analysis or POLO program, Yes you can find it by calculating on a dose response curve, dose on X axis and Mortality on Y, you can convert the values in log to find out minor differences. EC50 is the effective concentration at which you can find effectiveness of a bio-organic compound either in cell line treatment, microbial cell culture treatment or treatment of any cell preparation in culture. IC50 is the inhibitory concentration, which display that at this dose you get 50% inhibition of cellular activity, or inhibition growth up to 50%. For finding fiducial limit go for range of toxicity on upscale you will find 95% value on graph, or you can calculate it but that will not become original, It represents that 100-95=5/100=.05 level, either it may be significant or not.
I determine my IC50 and EC50 by curve fitting in the software Graphpad. If selected the program gives the fiducial limit at 95% CI for EC50.
There is a demo version of the software available with full functionality for 30 days.
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