Recently I synthesized the 3-substituted 3-amino oxindole starting from ketimines derived from isatin. After close examination, I come to know that the final product is in the racemic form. I want to find out the exact percentage of the individual isomer in each enantiomeric pair. Kindly suggest me a simplest and cost-effective method for this.
Hi Sameer,
Use peak area of one spectroscopic method on which peaks occur for both isomers .To fined out the percent , peak area for each isomer divided by total peak area for both isomers times 100.
Use HPLC with a chiral column for separation and area normalization for % isomers.
If you want to avoid chiral HPLC Column separation, you may try using a chiral shift reagent and get the ratio in NMR. If your enantiomers resolve, you can easily get the percent ratio.
Good luck
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