how to find out a Tumor cell lines whose p53 gene is not missing or mutated ?
Hello Weiping Huang
You can use the biological assay for p53 function. The critical biochemical function of p53, which is tightly linked with its tumor-suppressor activity, is the ability to activate transcription. The mutations observed in tumors abolish this activity, and so you can test the transcriptional competence using assays for p53 mutation. p53 can function as a transcription factor in yeast, and by performing the functional assay in yeast it is possible to take advantage of gap repair cloning techniques, which are highly effective with small amounts of unpurified PCR products.
In the article attached below the investigators present a simplified form of p53 functional assay in which yeast change color according to their p53 status, and they have shown that this assay is a rapid and simple means to detect p53 mutations in cell lines, peripheral blood lymphocytes, and tumors.
https://www.pnas.org/content/pnas/92/9/3963.full.pdf
You can also refer to the article below for more information on yeast functional assay to screen the p53 status of human cell lines.
https://pubmed.ncbi.nlm.nih.gov/9290701/
Best.
ATCC has a brochure detailing p53 mutation status in many of their cancer cell lines here: https://www.atcc.org/-/media/resources/brochures/tumor-cell-panels-brochure.pdf?rev=bd0ed144598143bab000c1434b0c201f
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