Recently i got a sequence from my lecturer then he ask me to found the mutation from it using bioedit, i've done this, i've alignment it with the normal sequences in NCBI, but i find so many mutation, i guess i was use the wrong technique, please tell me how to find it? I need it as soon as possible
It could be a trick question. How do we define "wild type" in a population with some diversity? If you have one or a few differences between two sequences, which is "normal" and which is "mutant". But maybe you should be viewing the sequences as amino acid (control-t to translate in BioEdit; but has to be in the right frame) and see if there is a mutation that introduces a stop codon.
Have you checked your sequence is in the correct orientation or does it need to be reverse complimented?
I will suggest you BLAST the nucleotide sequence (query) and download few, say 5 that have high seq. similarity with yours, and then you align using Bioedit. N:B if you still have higher variations, then you try to align the reverse complement of your sequence. I'm sure it's gonna work (I had a similar scenario).
- Badges
- Science topic
- Similar topics
- Higher Education
- Universities