Joseph Japhet

3 Questions 22 Answers 0 Followers

Questions related from Joseph Japhet

Can you align mRNA to a whole genome sequence of an organism, to check for sequence variations?

I'm carrying out a gene expression study (mRNA seq) of a gene, I noticed the BLAST results of the gene from species with same family had higher variations, but on aligning to the reference...

12 September 2022 7,109 5 View

Can I restart a PCR process in progress?

I started a PCR process and recall I didn't include the Primers of the gene of interest in the cocktail preparation process. The pcr was at the initial denaturing step (@1:00), Will my reaction be...

10 June 2021 1,128 7 View

Partial gene coding sequence translation

I sequenced a partial coding sequence (didn't run RACE) of a gene which got 800bp and the reference sequence got 1330bp. I have used bioinformatic tools such : Bioedit, Expasy, and ncbi orf...

01 January 1970 4,494 4 View