TEM images of the prepared silver nanoparticles samples look more aggregate compare to other TEM images.
Are there ways to measure aggregation level/degree of nanoparticles based on TEM images? Thank you
If you observe small-size and well-dispersed particles with repeated specific shapes in the TEM image of the nanoparticles, then you can say that you have the least aggregation or low level of aggregation.
Dynamic light scattering (DLS) would give you more relevant information, because, using TEM, you can never be certain that the aggregation you observe did not occur only during drying. Moreover, DLS is cheap and easy compared to TEM. Alternatively (if you have no access to a DLS setup), you could attempt to dilute (eg. 10 times) your samples. If you still observe the same difference in aggregation levels between your samples, there are good chance that, indeed, your samples have different aggregation states.
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