Where to order Tegosoft dec and ABIL WE09?

Hello everyone! Either I'm trying to setup an emulsion PCR, or design a new solar cream, but I do need to obtain these chemicals. I had contact with a company that asked me information about...

10 November 2018 2,779 2 View

Randomly well working PCR, how it optimize?

Hello, I'm trying to improve a PCR reaction. I sometime have one of the sample that get a "perfect" amplification, but most of them are just weak with primers left. It does not depend on the...

07 August 2018 7,409 11 View

No title

Hello, I find out the 3D structure of a protein I'm interested in. Then, I would like to highlight by color the part of the sequence I'm interested in. Let's say I study the sequence...

05 June 2018 1,834 3 View

[Bibliometry] What are the tags to be used on an article page in order to make them correctly analysed by scholar google?

03 April 2018 5,811 2 View

How to put two graphs side by side in a dev with different width?

I want 2 graph's, both 10 cm high, first 10 cm large and second 5 cm.

04 May 2014 3,303 3 View

Which diversity index do I use for calculating diversity from AFLP of plants with several ploidy level?

I'm doing analysis of phylogeography with a plant that can be either diploid, triploid or tetraploid. I get AFLP results. If I use Nei or Jaccard index there is a bias because a tetraploid has...

01 February 2014 6,179 0 View

How do you compute and analyse AMOVA with R (ade4)?

I have a dataset of binary data, and my PhD supervisor asked me to compute an AMOVA on it. I'm not familiar with such a test, furthermore the error messages are not convenient (private joke). So...

01 February 2014 4,137 8 View

How do I input data with Arlequin?

I was advised to use Arlequin to do some calculation such as AMOVA. After figuring out I couldn't launch it on OSX, I started it on Windows but now I just don't know how to put my data in. It is a...

01 February 2014 7,774 10 View

Who could send us some seeds?

A masters student of my lab is working on a plant named Honkenya peploides, it grows on beaches all around the polar circle and can also be found further south (Japan, Spain). We would like to...

06 July 2012 2,664 0 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View