How to find more commune repeated sequences from reads?

Hi all! I have a lot of reads that cover about all the genome of a species. I would like to find primers that are really often findable about them (and locate them in reads). I'm sure someone...

10 November 2018 8,214 6 View

Where to order Tegosoft dec and ABIL WE09?

Hello everyone! Either I'm trying to setup an emulsion PCR, or design a new solar cream, but I do need to obtain these chemicals. I had contact with a company that asked me information about...

10 November 2018 2,779 2 View

Randomly well working PCR, how it optimize?

Hello, I'm trying to improve a PCR reaction. I sometime have one of the sample that get a "perfect" amplification, but most of them are just weak with primers left. It does not depend on the...

07 August 2018 7,409 11 View

[Bibliometry] What are the tags to be used on an article page in order to make them correctly analysed by scholar google?

03 April 2018 5,811 2 View

How to put two graphs side by side in a dev with different width?

I want 2 graph's, both 10 cm high, first 10 cm large and second 5 cm.

04 May 2014 3,303 3 View

Which diversity index do I use for calculating diversity from AFLP of plants with several ploidy level?

I'm doing analysis of phylogeography with a plant that can be either diploid, triploid or tetraploid. I get AFLP results. If I use Nei or Jaccard index there is a bias because a tetraploid has...

01 February 2014 6,179 0 View

How do you compute and analyse AMOVA with R (ade4)?

I have a dataset of binary data, and my PhD supervisor asked me to compute an AMOVA on it. I'm not familiar with such a test, furthermore the error messages are not convenient (private joke). So...

01 February 2014 4,137 8 View

How do I input data with Arlequin?

I was advised to use Arlequin to do some calculation such as AMOVA. After figuring out I couldn't launch it on OSX, I started it on Windows but now I just don't know how to put my data in. It is a...

01 February 2014 7,774 10 View

Who could send us some seeds?

A masters student of my lab is working on a plant named Honkenya peploides, it grows on beaches all around the polar circle and can also be found further south (Japan, Spain). We would like to...

06 July 2012 2,664 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View