How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Can someone help me with tissue histology of mice?

I've been trying to assess the efficacy of a drug that I've purified in mouse model. I've induced mammary tumors in balb/c mice and treated them with my drug. I've harvested the tumors, and other...

16 December 2020 3,515 3 View

Can anyone help to get this research paper on OCB?

Turnipseed, D & Murkison, G (2000). Good Soldiers and Their Syndrome: Organizational Citizenship Behavior and the Work Environment, North American Journal of Psychology 2(2)

11 November 2020 7,488 3 View

What are some landmark texts and papers on agroecology we need to follow for a clear understanding?

We often read a lot about sustainable agriculture or conservation agriculture (or recently, climate-smart agriculture) but it is often difficult (especially for early-stage researchers) to fathom...

08 April 2020 9,212 10 View

Can anyone share me a better image of the attached map? In which book the map was originally published?

The map is reproduced in Eaton, Richard M. The Rise of Islam and the Bengal Frontier, 1204-1760. Berkeley: University of California Press 1993; reference given are: Barrie M. Morrison,...

13 August 2019 1,485 2 View

Does 4T-1 cells induce ascites in mice?

I am new to in vivo research. I have tried to induce solid tumors in mice by injecting 4T-1 cells in the mammary fat pad, which is an established procedure. While i mostly ot solid tumors, 2 or 3...

18 June 2019 973 3 View

How to solve problems arising from choosebank() command in R?

I am using seqinr package in R. But when I am giving the command choosebank() to find out the existing databases or commands like choosebank('swissport") to open any specific database, R is...

15 April 2019 1,541 3 View

What is the scope of Fortran in machine learning?

I intend to use ML for molecular dynamics simulations and want to know of packages available in Fortran. Would it be more efficient to use some other language instead for the long run?

09 January 2019 8,295 2 View

Which regression/multivariate technique to apply if dependent var is continuous scale, cant be categorised, non-normal, Indep Variables dichotomous?

02 September 2018 1,981 2 View

For droplet-based sc-RNA sequecing data, library size varies across cells. How does one tackle this when calculating statistical variance for genes ?

To calculate the statistical quantities like variance or mean expression of a gene across cells, it is unfair to compare expression counts of a say gene A, if we know different cells has not be...

14 July 2018 10,016 2 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

When making a phylogenetic tree, do I need to concatenate the chromosome and the plasmids for my reference?

This is such a basic question but I come from a non-bioinfo group so I keep getting different answers. I was wondering if when making a phylogenetic tree whether you have to concatenate the...

14 January 2021 2,492 2 View