How to find antigenicity of a protein ? any particular software?

More Mohsen Sarikhani's questions See All

Focus on water use optimization or water resource optimization which one is effective way toward sustainability?

Water resources are limited and have been significantly reduced by pollution due to urbanization in recent decades. Therefore, water resource optimization only highlights the ways these resources...

27 July 2024 9,303 1 View

How to do Data Farming and Surrogate Model?

Dear Scholars How is it possible to define an algorithm for data augmentation and use a surrogate model? In particular, I want to use data farming to create artificial data instead of real data...

12 June 2024 2,484 4 View

Hi please how can i find quasi- experimental sample size calculator to compute the exact number ?

i need sample size calculator for quasi study design

02 June 2024 9,822 2 View

Hello drs Im asking about new topics in orthodontic?

hello drs can you help me finding new topics in orthodontic for PhD research, i preferred clinical topics specially using CBCT

30 April 2024 9,258 3 View

Is there a criterion for choosing the secondary outcome or the second dependent variable?

In an interventional study, is there a criterion for choosing a secondary outcome or a second dependent variable? Should these two variables in the study of medical sciences and especially...

26 April 2024 9,695 1 View

How long does e.coli survive on filter paper at room temperature?

I have been sent e.coli strains on filter paper and after 10 days I have not received them yet. I am wondering how long would the bacteria survive. Thank you in advance

21 April 2024 881 0 View

Re perform PCR reaction on the same first one for genotyping?

I performed genotyping analysis. Some samples give a result of one SNP but didnt give a result for another SNP. Note, these SNPs are performed at the same plate (same condition). Depending on the...

26 March 2024 7,013 0 View

Is the behavior of carbon steel (e.g., S275) under tension-compression tests symmetric?

Dear colleagues I am organizing tension tests on some existing steel elements (e.g., S275). I will extract the yielding strength and ultimate strength of the existing steel elements. I believe...

13 March 2024 2,035 0 View

How to calibrate temperature on a Peqlab Primus 25?

We have this machine and the temperatures are not correct. Does anyone know how to calibrate it? Thank you in advance

05 March 2024 5,895 2 View

Evaluation of seismic resilience of steel structures?

Question about how and types of methods to optimize steel structures for seismic resistance

29 February 2024 7,569 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

Looking for a bioinformatics expert especially one who has expertise in Nanoparticles docking ?

Silver Nanoparticles docking with alpha amylase and alpha glusidase .

11 July 2024 6,883 2 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View

Biswapriya Biswavas Misra

Hello, if you reply of in silico prediction tools for leads (rather than literature on TNF alpha a specific source), then the following would help.

There are several you can find on line:

1. http://imed.med.ucm.es/Tools/antigenic.pl

2. http://sysbio.unl.edu/SVMTriP/prediction.php

3. http://tools.immuneepitope.org/tools/bcell/iedb_input

4. http://bioinformatics.org/JaMBW/3/1/7/

But, I have always successfully used:http://www.imtech.res.in/raghava/lbtope/protein.php

Thanks,

Biswa

Mohsen Sarikhani

thank you