How to filter reads in fastq for small RNA?

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How do I write a single author review article and where to publish it?

I am interested in writing a review article but don't know where to start. Any advice would be welcome.

10 June 2024 5,515 1 View

How to add new papers?

17 March 2024 2,330 1 View

InderScience submission Help?

In the InderScience submission, I need the names and details of 4 international experts (I am from India, so at least 2 must be from outside country) I am trying to submit an article that is based...

11 March 2024 9,402 0 View

From where can I obtain 5beta-pregnane-3,20-dione standard for HPLC?

Can anyone please suggest how can I procure 5beta-pregnane-3,20-dione standard? I have been searching different companies, mostly I have found the 5alpha form of the same chemical. I am currently...

19 November 2023 4,085 2 View

When people don't own Mercedes car or BMW car then why do they buy merchandize (eg: t-shirts, keychains) of these brands??

Many luxury brands in automobile sector offer their merchandize and many people buy them. What motivates these consumers? Is it just the brand association ?

17 August 2023 7,167 1 View

Why i am getting no recovery of chitin from fungal biomass?

I get good amount of fungal biomass in submerged fermentation but no chitosan get extracted after completing the extraction process using NaOH treatment. Please help

03 August 2023 3,952 1 View

Can i use ammonium persulphate or ammonium ferrous sulphate for protein precipitation?

as we normally use ammonium sulphate for the same

14 July 2023 4,607 3 View

How to conduct self discharge test for supercapacitor with/without Potentiostat?

A gel polymer based supercapacitor device is fabricated and need a testing for self discharge. I am using CHI instrument for the electrochemical characterization. Also, the leakage current and...

31 May 2023 8,912 1 View

How to calculate transference number for gel polymer electrolytes?

Also, the transference number and transport number are same or different? If they are different then how to calculate them for cations and anions?

31 May 2023 3,753 1 View

How can we determine hydrogen bond strength using FTIR data? We have seen researchers using MHBS calculations? Is it accurate?

MHBS should be a unitless quantity?

28 May 2023 9,262 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View