How to prevent Band/Edge Pass filter emission on Pulsed Laser excitation?

We are observing emission from both the Edge and the Bandpass filters on excitation with a Pulsed Laser. The emission is significantly high with multiple/uncontrolled peaks superimposed on the...

01 December 2020 5,449 1 View

How to take the layer of glass in solar cell?

I'm willing to re-create a perovskite based solar cell but it is having glass in it and I don't know how to take glass in silvaco atlas.

06 May 2020 6,238 12 View

Is log i vs log v (scan rate) an appropriate parameter for defining the capacitive and battery behaviour.?

According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...

26 February 2020 4,592 4 View

Hi I am using Hofstede VSM 94 model and bit confused in calculating the different dimensions among regions??

Please provide me with a example to compute it.

16 September 2019 3,423 2 View

In which solvent CdS-TiO2 composite may dissolve?

want to perform CV measurement.

14 August 2018 5,773 2 View

Arabidopsis leaves are not bleaching in high salt concentration?

Hi there, I am doing Arabidopsis leaf disc senescence assay by using 300mM and 500mM NaCl solution. After punching leaf discs, I am keeping them in distill water (Control), and in different salt...

07 June 2018 9,061 3 View

How to do ChIP qPCR data analysis to study Histone modification?

06 February 2018 6,839 2 View

Why do bands stay on sds page gel even after performing western blot?

I have performed transfection of n and p genes of ppr virus in vero cells.. Later i went for sds page followed ny westwrn blotring... After staining my page gel i could still see some bands on my...

24 January 2018 4,202 9 View

Why can't mammalian proteins be observed in sds page?

I'm trying to transfect N and P proteins of PPR virus in mammalian expression system. Why can't the proteins be viewed on sds page, why is western blotting necessary to observe the expressed protein?

23 January 2018 8,139 2 View

Water molecule starting at atom 69515 can not be settled. Does anyone have an idea of this in GROMACS ?

03 July 2016 6,560 26 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View