Hello,
I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended by the manufacturer, with 100 mM glycine pH 2 for 5 minutes. Unfortunately, I washed off also impurities together with the antigen...Do you know how could I obtain pure antigen? Or do you know about alternative fast methods to extract an analyte from human serum?
Thanks in advance,
Veronica
You could do that by the liposomes method: Article Direct extraction of A and B blood group antigens from human...
you could also use monoclonal antibodies method:Article The chicken erythrocyte-specific MHC antigen. Characterizati...
But co-immunoprecipitation method is works best for me. The method provides a rapid and simple way to separate a specific protein from a sample containing thousands of different proteins, such as serum, cell lysate, homogenized tissue or conditioned media.
Thank you Maurice. What do you mean for co-immunoprecipitation? The immunoprecipitation exploiting another protein interacting with my antigen?
If you know the isoelectric point of the protein and have access to the equipment then isoelectric focussing both concentrates and separates protein very effectively
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