False formulated question!!

Do you want to extract BEFORE or AFTER   200 W irradiation.

When you write :

"in order to study the change of molecular structure of the dye that has been encapsulated."

Your formuation is very obscure. Please refine  the part (see above) of your question and send infos.

here the copy of my answer implemented in another question:

Adam, Christopher and Guido posted valuable and helpful answers.

Several monthes ago, you posted  2 questions  and received valuables answers too.

I give you again following hints:

Start  ggogle scholar  (ipse dixit)  and don 't collect our answers  and give comments to our answers.

I answer your new question :

How to extract fluorescence dye encapsulated in silica shell

as follows:

Do you want to extract the Dil dye before or after  200W irradiation?

The extraction of the dye depends (prior irradiation ) of counter anion and partition coefficient of the dye in the chosen solvent. Answer my question about the counter anion and try the solubility of the dye (alone)  in various solvents  (from petrolether to water). You can crush the silicagel  and try to extract in a vessel with ultrason instead magnetic stirring.

After irradiation you 'll extract a mixture of Dil dye and destroyed dye. You can investigate the by-products with TLC (thinlayer-chromatography)

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How to extract fluorescence dye encapsulated in silica shell

4

What affect the increasing of fluorescence intensity upon exposure to light?

3

Method to detect molecular structure

JRG

This paper seems to have a lot of methods that would be useful for your project:

Dissolution behaviour of Fluorescent Silica Nanoparticles in

Biological Conditions

I couldn't find a link to the main paper, but here is the supplementary methods section:

http://www.rsc.org/suppdata/cc/c2/c2cc34023b/c2cc34023b.pdf

Here is a review on drug delivery by mesoporous silica nanpoarticles:

https://www.researchgate.net/profile/Jessica_Rosenholm/publication/45800584_Rosenholm_JM_Sahlgren_C_and_Linden_M_Towards_multifunctional_targeted_drug_delivery_systems_using_mesoporous_silica_nanoparticles-opportunities_challenges_Nanoscale_2_1870-1883/links/09e4150f4129875efd000000.pdf

Here is another paper that should be of interest:

Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies.

https://www.ncbi.nlm.nih.gov/pubmed/22767252

Article Towards multifunctional, targeted drug delivery systems usin...

Very good answer posted from Adam. Here is the link of Adam

Here is another paper that should be of interest:

Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies. 

(Ipse dixit Adam)

https://www.ncbi.nlm.nih.gov/pubmed/22767252

via Google Scolar, I obtained the same infos with abstract and picture.

http://pubs.rsc.org/en/content/articlelanding/2012/cc/c2cc34023b#!divAbstract

Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies

Here we show that commonly employed Stöber type fluorescently labelled silica nanoprobes degrade by hydrolytic dissolution, accelerated under biological media conditions as compared to water alone. We have thus developed a method to greatly improve their stability under such conditions.

Eugene Mahon,*a Delyan R. Hristova and Kenneth A. Dawson*a

Show Affiliations

Chem. Commun., 2012,48, 7970-7972

DOI: 10.1039/C2CC34023B

Received 04 Apr 2012, Accepted 25 Jun 2012

First published online 26 Jun 2012

However the pdf is very expensive and not free of charge in internet. Please contact the library of your uni

JRG