PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View

When will we have access to Brazilian agricultural censuses microdata publically available? Can Google and Microsoft help it?

Microdata are a wonderful way of analyzing specific issues in an heterogeneous environment. Brazil is wonderfully diverse country, and its countryside is also extremely heterogeneous. We have...

25 October 2020 8,900 2 View

Should I use anesthetics before guillotine If I inteed to see brain inflammation?

Hello, I also want to do some biochemical analyses and need to take some blood of the rats. To take the blood I need to use anesthetics. But I want to evaluate TNF-a and oxidative stress in the...

19 October 2020 3,835 3 View

How to download citation network data from Web of Science (WoS)?

I want to do citation analysis on papers on ecnomics, but I don't know exactly how to get this data from Web of Science, though many sources say this is one of the very good datasets in this area....

16 March 2020 4,184 4 View

What is a good dataset and technique for citation analysis in Economics?

I would like to track the presence of some ideas in papers in economics, and I have been reading about citation network analysis and I have found some datasets (like the...

26 February 2020 2,848 1 View

What about the use and interpretation of wavelets, any suggestions for articles or books for me to delve into?

What about the use and interpretation of wavelets, any suggestions for articles or books for me to delve into?

13 March 2019 1,788 2 View

Any alternative methods to administer substances in rats?

Hi! I'm doing a study with Wistar Rats and the concentration in humans is 30g/day. I used an average human weight of 60Kg, therefore, that makes the concentration in humans 500mg/Kg/day. When i...

09 August 2018 6,466 6 View

How can I quantify an enzymatic kinetics that converts ATP to AMP? What techniques besides HPLC can I use?

How can I quantify an enzymatic kinetics that converts ATP to AMP? What techniques besides HPLC can I use? Thank.

10 December 2017 9,952 5 View

Good books on the history of the HIV epidemic?

Does anybody knows a couple good books on the history of the HIV epidemic and first outbreak response? Trying to trace some parallels and I believe that as I was not born at that time some good...

19 April 2017 8,956 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View