I have a material supporting a peroxidase enzymes and I wish to collect the infrared spectrum of the complex support-enzyme, but this system is in buffer medium (citrate-phosphate). The technique available for FTIR analysis is by KBr pellet therefore what is the pre-treatment useful to dry the support with enzyme without degradation of biomolecules attached?
11/24/22
Dear Arthur,
One thing you can try is to dialyze the enzyme vs. DI-water to remove the buffer constituents, then dry the support-enzyme by lyophilization. You may then be able to mix the dry residue w/ KBr to produce a pellet for FTIR analysis. This assumes that the support-enzyme complex survives the dialysis step.
I hope this information helps you.
Bill Colonna Iowa State University, Ames, Iowa, USA [email protected]
Experiment this technique:
1. Dialyze the sample against a suitable KBr solution.
2. Expose the sample to evaporation under vacuum at low temperature.
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