PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View

Since I am design automatized grader for forest seeds, it is important for me to know the following?

1. Is there a difference between a dormant seed, for example, of Scots pine, a fossilized dead seed, and an active live one by spectrometric or other characteristics? 2. Is it possible to...

26 November 2020 9,275 2 View

Should I use anesthetics before guillotine If I inteed to see brain inflammation?

Hello, I also want to do some biochemical analyses and need to take some blood of the rats. To take the blood I need to use anesthetics. But I want to evaluate TNF-a and oxidative stress in the...

19 October 2020 3,835 3 View

How does annealing affect the crystallinity and hardness of a blended wax product?

Dear all, I'm hoping to receive some feedback and tips so that I can better understand the behaviour of a material I am working with: The material is a blend of paraffin and microcrystalline...

14 August 2020 1,605 3 View

How to extract data Night Light?

24 March 2020 8,301 2 View

What is the best way to interpret and analyze a phylogenetic tree built from categorical functional traits ?

I have built a phylogenetic tree using categorical functional traits, such as pollination and dispersion syndromes, phenology, etc. Now, I can see the dicotomies and the points in which the plant...

24 February 2020 6,051 3 View

Can someone help with a creep in steel under fire conditions modelling in Ansys APDL?

Hello everyone. I am encountering some problems while chasing after the convergence of my model. I am using Ansys APDL in order to model the creep in steel under high temperatures. I already did...

06 January 2020 575 3 View

Does phase 2 clinical trials need a superiority margin?

23 July 2019 5,158 3 View

No title

I am developing a research, using as a method the modeling of structural equations, which aims to identify the determinants of sustainable consumption, or conscious consumption. However, I am...

24 November 2018 7,287 10 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

As the first flagelant confraternity is mentioned one in Perugia in 1260. But, some data testify such confraternity in 1214/Zadar and 1215/Dubrovnik?

Data from Zadar's archive mention the fraternity of flagellants (dei flagellanti) in Zadar in 1214founded in the church of St. Silvester (fratalia seu scola sancti Silvestri Iadere Frustancium)....

14 February 2021 3,136 2 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View