Is it possible to use Cas9 (WT) to perform CRISPR-screening with a sgRNA subpooled library (Addgene catalog number: 83971) designed to be used with dCas9 systems? Beyond mutations at nuclease domains (HNH and RuvC), is there any other difference between Cas9 and dCas9 enzymes that could lead to impairment of sgRNA recognition by Cas9 (WT)?

Thanks!