How to extract bacterial and virus antigen from infected leaves?

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

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How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

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Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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What is the most suitable server for predicting protein structures in bacteria?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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Are protein samples in SDS compatible with ELISA?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

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Rajeshkumar Natwarlal Vadgama

You can use the protein extraction procedures as it is important to separate Protein from other biomolecules like carbohydrates and lipid. In next step, you can go ahead with antigen purification using antigen's specificity. You may have antibody specific to your antigen and so you could use some separation methodology based on antibody specificity. May be chromatographic or ELISA techniques may help you.

Divya Bhanu Sharma

Thank you very much sir.

Aamal Ghazi Mahdi Al-Saadi

Hi,

please see here: http://www.sciencedirect.com/science/article/pii/S0166093415003195

Mohamed Gadallah

Please find the article below

Thank you very much. i have also found these papers helpful.