I need bacterial and virus antigen from bacteria and virus infected leaves, respectively. Can i use any protein extraction method for this purpose irrespective of pathogen type? I am confused that extracted proteins from infected leaves will also have proteins from leaf along with antigen. Can i use this mixture without purification of antigen proteins?
You can use the protein extraction procedures as it is important to separate Protein from other biomolecules like carbohydrates and lipid. In next step, you can go ahead with antigen purification using antigen's specificity. You may have antibody specific to your antigen and so you could use some separation methodology based on antibody specificity. May be chromatographic or ELISA techniques may help you.
Hi,
please see here: http://www.sciencedirect.com/science/article/pii/S0166093415003195
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