How I can reduce the cost of new antibody production?

I need new antibodies for different phospho sites for a human protein of my interest. I am contacting different companies. Polyclonal antibody production in rabbit host is expensive. Is it OK if I...

31 December 2019 3,032 4 View

What are the best markers (genes) to determine different DNA damage responses?

After drug treatments I would like to check a number of genes by qPCR to determine involvement of different DNA damage pathways namely homologous recombination (HR), NHEJ, MMEJ etc. Which genes...

02 March 2018 4,046 5 View

Is there well established cell line for primary peritoneal carcinoma (PPC)?

01 February 2015 9,282 2 View

How is ALDH1A1 related to cancer drug resistance?

31 December 2014 8,074 2 View

How to evaluate/validate primary cancer cell line?

I have isolated primary cancer cells from ascites. I would like to know the proper way to evaluate/validate primary cancer cell line before any kind of study. I just know that I need to confirm...

01 February 2014 7,730 1 View

Does IC50 value reduce as the passage number of cell increases?

In SKOV-3 ovarian cancer cell line I determind the IC50 value for certain drug at passage number 6. However, at 36th passage I am getting IC50 value about half of previous one. Is that usual? The...

10 November 2013 8,217 10 View

What/'s the role of endoplasmic reticulum during cell division?

Endoplasmic reticulum (ER) contains large amount of calcium ions. During cell division does it release all its content and break down? As it is a continuous membrane how is it distributed in two...

06 July 2013 7,934 3 View

Do I need to filter after dissolving drugs in DMSO?

I am preparing drug stock in DMSO. To take the weight of drug I need to expose it outside the cabinet. Thus I am wondering about microbial contamination. Do I need to filter it with 0.22um syringe...

06 July 2013 9,489 19 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View