Md. Hafiz Uddin

9 Questions 15 Answers 0 Followers

Questions related from Md. Hafiz Uddin

How I can reduce the cost of new antibody production?

I need new antibodies for different phospho sites for a human protein of my interest. I am contacting different companies. Polyclonal antibody production in rabbit host is expensive. Is it OK if I...

01 January 2020 3,083 4 View

What are the best markers (genes) to determine different DNA damage responses?

After drug treatments I would like to check a number of genes by qPCR to determine involvement of different DNA damage pathways namely homologous recombination (HR), NHEJ, MMEJ etc. Which genes...

03 March 2018 4,102 5 View

Is there well established cell line for primary peritoneal carcinoma (PPC)?

PPC is a rare type of cancer having extensive similarity with ovarian epithelial cancer. I am dealing with primary cell line of PPC and looking for well established one for comparison. Is it a...

02 February 2015 9,347 2 View

How is ALDH1A1 related to cancer drug resistance?

In many cancer, ALDH1A1 found to be associated with chemoresistance. Does anyone has comprehensive understanding on the molecular mechanism of ALDH1A1 mediated chemoresistance? I just found an...

01 January 2015 8,133 2 View

How to evaluate/validate primary cancer cell line?

I have isolated primary cancer cells from ascites. I would like to know the proper way to evaluate/validate primary cancer cell line before any kind of study. I just know that I need to confirm...

02 February 2014 7,786 1 View

Does IC50 value reduce as the passage number of cell increases?

In SKOV-3 ovarian cancer cell line I determind the IC50 value for certain drug at passage number 6. However, at 36th passage I am getting IC50 value about half of previous one. Is that usual? The...

11 November 2013 8,280 10 View

What/'s the role of endoplasmic reticulum during cell division?

Endoplasmic reticulum (ER) contains large amount of calcium ions. During cell division does it release all its content and break down? As it is a continuous membrane how is it distributed in two...

07 July 2013 7,996 3 View

Do I need to filter after dissolving drugs in DMSO?

I am preparing drug stock in DMSO. To take the weight of drug I need to expose it outside the cabinet. Thus I am wondering about microbial contamination. Do I need to filter it with 0.22um syringe...

07 July 2013 9,630 19 View

How to enrich side population? What percentage of SP lose their stemness after a few days culture?

As SP isolation is difficult, I am looking for a suitable technique to enrich it. Any suggestion or comments will be appreciated.

05 May 2013 1,710 1 View