How I can reduce the cost of new antibody production?

I need new antibodies for different phospho sites for a human protein of my interest. I am contacting different companies. Polyclonal antibody production in rabbit host is expensive. Is it OK if I...

31 December 2019 3,032 4 View

What are the best markers (genes) to determine different DNA damage responses?

After drug treatments I would like to check a number of genes by qPCR to determine involvement of different DNA damage pathways namely homologous recombination (HR), NHEJ, MMEJ etc. Which genes...

02 March 2018 4,046 5 View

Is there well established cell line for primary peritoneal carcinoma (PPC)?

01 February 2015 9,282 2 View

How is ALDH1A1 related to cancer drug resistance?

31 December 2014 8,074 2 View

How to evaluate/validate primary cancer cell line?

I have isolated primary cancer cells from ascites. I would like to know the proper way to evaluate/validate primary cancer cell line before any kind of study. I just know that I need to confirm...

01 February 2014 7,730 1 View

Does IC50 value reduce as the passage number of cell increases?

In SKOV-3 ovarian cancer cell line I determind the IC50 value for certain drug at passage number 6. However, at 36th passage I am getting IC50 value about half of previous one. Is that usual? The...

10 November 2013 8,217 10 View

Do I need to filter after dissolving drugs in DMSO?

I am preparing drug stock in DMSO. To take the weight of drug I need to expose it outside the cabinet. Thus I am wondering about microbial contamination. Do I need to filter it with 0.22um syringe...

06 July 2013 9,489 19 View

How to enrich side population? What percentage of SP lose their stemness after a few days culture?

As SP isolation is difficult, I am looking for a suitable technique to enrich it. Any suggestion or comments will be appreciated.

04 May 2013 1,653 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View