How to eliminate hump in HPLC peaks for plant extracts separation?

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ICP-calculation-how to calculate dilution factor?

in the formula given Conc.in ppm x Volume in mL x Dilution Factor X 10-4 Wt % = -------------------------------------------------------------------------...

24 July 2022 5,000 0 View

What type of regression analysis to use for data with non-normal distribution?

What is the non-parametric version of multiple regression. my data set is highly skewed

01 March 2022 4,575 33 View

How the calcination temperature varies for hydrothermal and solid state synthesis?

need detail explanation.

01 February 2020 5,006 2 View

Hidden Markov with Gaussian mixture : (1) No of Hidden States & Gaussian Mixture selection ? (2) Any specific distribution of my i/p data look like?

I would like to clarify two queries during training of Hidden Markov model Data set background I have 2 list say St & Rt. Length of my list len(St) = 200 & len(Rt) = 100 Each element in...

29 May 2019 6,876 11 View

Quantum Gravity or General relativity?

I would like to do work on quantum gravity. But general relativity is not complete. So if i want to do work on GRT. I am beginner for this course. GRT fails in few aspects. Any one suggest me...

23 March 2019 2,976 12 View

Raw score or recoded values for chi-square test?

I am using chi-sq to find out the relationship between the nominal variable gender and key variables family environment and vocational identity measured on 5 point likert scale. Should i select...

07 March 2019 3,906 3 View

Which simulation tools is suitable for simulate cognitive radio networks ?

The simulation tool whether support real time implementation for user.

27 October 2018 5,998 9 View

Would it be o.k. to convert a dichotomous True False scale to a 5 point likert scale for factor analysis among 3 scales 2 being 5 point Likert ?

I am using short form of family environment scale(2 point) and two 5 point scales for measuring life skills and vocational identity

31 October 2017 1,796 3 View

What is double way of unification in Physics?

Prof.Hawking says that Theory of Everything is possible in two ways. what are they?. I think unifying four interactions or unifying quantum mechanics and general relativity. Is this correct?.

01 March 2016 4,718 8 View

Are there any characteristic energy scale for quarks?

Pls Answer me.(Particle Physics People)

02 February 2016 2,327 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Detlef Jensen

Dear Ayyamperumal Esakki

I am afraid to say, but without any details about your experiments, samples, components, conditions a question like this simply cannot be answered.

Please provide the essential information we need to understand your issue, evaluate the situation and to come up with some suggestions.

Thank you

Detlef.

Jasim Al-saadi

Try to increase the strength of your M.P

Navid J. Ayon

Provide details about the problem you are having and the conditions you are using and then the experts can help you sorting this out. I often get lots of insights from thia platform which truly helps in research.