I am currently designing primers against conserved regions and in my sequence I have many "N" (undetermined nuecleotides like...AGTCNNNTCNNNATNA"). Help me How can I chnage these "N" to respective nucleotide s using bioedit or any other software.
Hi Zulfikar
in Bioedit, to change the nucleotide us should change the option from "Select/Slide" to "Edit" at Mode, under the Save symbol, upper left. Choose either overwrite or insert then change the N base.
With Bioedit, select edit in mode. click on the sequence you need to change and then make your change. Have you tried Ridom TraceEdit. Maybe you should look at it.
Agi Vivian Hasan Nasrullah actually when I open sequence in bioedit it doesnot open as peaks..do you have any idea why is that so? and manually how would I know where to place suitable nucleotide.
Have you tried opening with chroma's. It opens as peaks. Or better still Ridom TraceEdit. With these you'll know exactly where to place suitable nucleotides.
Have you tried opening with chroma's. It opens as peaks. Or better still Ridom TraceEdit. With these you'll know exactly where to place suitable nucleotides.
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