I want to perform morphological analysis of callus cell through staining. But the problem I ma facing is to obtained thin layer of callus cells which allow clear observation. Is there any manual method to obtain thin layer of callus cells?
Dear Fizzah,
If you are planning to get thin sections of the callus you must process with some plant anatomical techniques in order to fix and embed your samples. You can do it with paraffin or historesin.
Have a look on this manuscript:
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005
On the other hand, it seems easy to get the access to the callus cells and also do different staining methods after you just press the callus between the glass slide and coverslip.
Please let me know if I can help you in a more specific way.
Best regards,
João
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