Can I use 2.5M sodium hydroxide solution to adjust the ph of potassium phosphate buffer for hplc analysis?
My column is 150mmx4.6mm, 5um.
I discovered from real-time experiments as a young researcher earnestly working on zerovalent iron that the product yield from the green synthesis of nanoparticles using plant extract is always...
19 May 2024 5,605 6 View
For the removal of heavy metals from an aqueous media, how long should wastewater could be kept before carrying out heavy metals adsorption?
17 January 2024 3,300 5 View
I found in our store, a number of HPLC grade acetonitrile bottles which exceeded the normal shelf life (with about three years!). Do you have any idea of what the possible problems that may...
13 October 2023 8,527 2 View
I have been running a batch of samples with the hplc machine and I have noticed that each time I run another batch of samples, the retention time of the peaks shift backwards. For example, a week...
23 August 2023 6,594 3 View
It sounds that the iodine mine activities has negative effect on rain amount, but i haven"t any article for this theory. I wonder someone help me.
11 August 2023 9,786 0 View
For LOD and LOQ I need a *blank sample* and *sample of known concentration*. For the *blank sample*, should I just fill the vial with my diluent only ? And for *sample of known concentration*,...
26 July 2023 7,801 2 View
What is the recommended approach for early researchers in Educational Technology: Should they narrow their research focus on a specific topic, such as Learning Management Systems (LMS) for...
20 July 2023 6,735 4 View
I'm currently doing design of experiments to optimize my hplc method. The 3 response parameters for my design of experiments are Peak retention time, resolution and asymmetry. For some of my...
14 May 2023 946 2 View
I'm currently analyzing a drug using hplc. I've been using the same method the whole time. The flow rate has been 2ml/min and the pressure was usually around 170 bar. The mobile phase is methanol...
20 March 2023 2,168 2 View
Does changing the concentrations of your mobile phase affect the peak you obtain? I was initially using methanol:phosphate buffer 80:20 % and I obtained good peaks. I'm currently doing design of...
08 March 2023 7,934 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View