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Questions related from Damola Ajani
I found in our store, a number of HPLC grade acetonitrile bottles which exceeded the normal shelf life (with about three years!). Do you have any idea of what the possible problems that may...
14 October 2023 8,498 2 View
I have been running a batch of samples with the hplc machine and I have noticed that each time I run another batch of samples, the retention time of the peaks shift backwards. For example, a week...
24 August 2023 6,567 3 View
For LOD and LOQ I need a *blank sample* and *sample of known concentration*. For the *blank sample*, should I just fill the vial with my diluent only ? And for *sample of known concentration*,...
27 July 2023 7,761 2 View
Can I use 2.5M sodium hydroxide solution to adjust the ph of potassium phosphate buffer for hplc analysis? My column is 150mmx4.6mm, 5um.
11 June 2023 8,933 0 View
I'm currently doing design of experiments to optimize my hplc method. The 3 response parameters for my design of experiments are Peak retention time, resolution and asymmetry. For some of my...
15 May 2023 804 2 View
I'm currently analyzing a drug using hplc. I've been using the same method the whole time. The flow rate has been 2ml/min and the pressure was usually around 170 bar. The mobile phase is methanol...
21 March 2023 2,130 2 View
I am doing hplc analysis and recently purchased a new column because the old one has degraded. Can one simply install the new column and start straight away with running standards or is there a...
09 March 2023 3,447 4 View
Does changing the concentrations of your mobile phase affect the peak you obtain? I was initially using methanol:phosphate buffer 80:20 % and I obtained good peaks. I'm currently doing design of...
09 March 2023 7,905 1 View
