What is the best method to quantify an IHC?

I know tat ImageJ has options to count cells either manually or automatically but my protein of interest lights up the synapses and hence can't use both. I thought of measuring the mean grayscale...

04 November 2016 4,710 2 View

Can non-neutralising antibodies reduce the viral load in-vivo?

A neutralising antibody is one, which in an in-vitro neutralisation assay reduces the viral infection in its presence. This is because it hinders in the infection process. (correct me if wrong) On...

14 August 2016 4,472 8 View

How valid is PPNC (percentage predicted neck circumference) in different populations?

We are doing a prevalence study on Obstructive sleep apnea (OSA). There is an established association with neck circumference. Neck circumference increases with height and there is a variable...

05 July 2016 9,171 3 View

Why doesn't hypertonic sucrose used as a cryoprotectant change cellular architecture/shape?

Sucrose acts as a cryoprotectant by removing the water (by exosmosis?) Does this alter the size of the cells? Technically the cells should shrink due to the 30% sucrose hypertonic solution being...

27 June 2016 4,281 3 View

Is there a substitute for DTT in plant protein extraction?

Most protocols suggest the use of DTT (Dithiothreitol), a reducing agent in the isolation QB buffer for extraction of protein from plant tissue. I plan to run an SDS-PAGE gel. Is there an...

20 June 2016 668 4 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Does the UV radiation affect on fruits ?

I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...

24 January 2021 8,350 20 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Can I make a meta-analysis with different variables in the same forest plot?

14 January 2021 1,733 4 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View