Does FACS of suspended cells from brain tissue work with cell surface epitopes?

26 October 2023 1 9K Report

I am planning to run flowcytometry on cell suspensions from brain tissue to look for cells that bind to certain neuronal surface antibodies.

1. Do existing dissociation methods work for adult rodent/human brain tissue?

2. Do the dissociation methods preserve the cell surface antigens intact to do FACS?

Louis-Charles Béland

Hello,

Many different protocols exists to prepare single cell suspensions from fresh brain tissue. They usually require both mechanism and enzymatic digestion followed by Percoll gradient isolation. These protocols are usually time consuming. Check the methods in PMID: 33846226 (done is spinal cord but will work for brain)
Dissociation methods will preserve most of cell surface antigen

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