I performed an invasion assay with mutants so I have control and wild type and several other mutants. I want to do one way ANOVA in SPSS I am not sure what should be my dependent or independent variables? I am very confused.
Thank you in advance
I am trying to infect EO771.lmb cells with lentivirus to express luciferase and Tdtomato. My cells are not expressing anything. I tried spinning the virus for 90 min with media+polybrene, but it...
08 January 2023 2,341 1 View
Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...
31 January 2021 2,994 2 View
Hello, I am doing a cancer metastasis experiment both primary and experimental metastasis. I would like to know has anyone collected CTC from mice blood and how did you store it? Regards Tasnim
08 March 2020 3,150 2 View
Hello, I have a 231 cell line growing but it the media is getting cloudy periodically. I have changed the media, PBS, trypsin but still it is getting contaminated. I thought it might be...
08 September 2019 831 4 View
08 September 2019 8,284 4 View
Hello, I am trying to circularize DNA which is 9000 bp long. I am using the thermofisher T4 ligase and their self circulation protocol but I am not getting any colonies. Even if I get one colony...
30 July 2019 8,830 10 View
Hello, I have a plasmid and I have deleted a section containing an undesired DNA. I am producing a control plasmid with everything but the gene of interest. So I do not know how to close the...
05 June 2019 8,051 3 View
I have a plasmid of 11242 bp I want to cut out a chunk of it encoding a protein I don't need around 2000 bp. what would be the best way to do that?
01 February 2019 8,256 9 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View